Title : Protein-Ligand Fishing in planta for Biologically Active Natural Products Using Glutathione Transferases.

Pub. Date : 2018

PMID : 30510558






1 Functional Relationships(s)
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1 Following transient and stable expression in Nicotiana and Arabidopsis, respectively, the GSTs were recovered using Strep-Tactin affinity chromatography and the bound ligands desorbed and characterized by LC-MS. AtGSTF2 predominantly bound phenolic derivatives including S-glutathionylated lignanamides and methylated variants of the flavonols kaempferol and quercetin. Quercetin glutathione S-transferase PHI 2 Arabidopsis thaliana