Title : The use of a specific fluorescence probe to study the interaction of adrenodoxin with adrenodoxin reductase and cytochrome P-450scc.

Pub. Date : 1987 Jul 25

PMID : 3038861






2 Functional Relationships(s)
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Protein Name
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1 Addition of adrenodoxin reductase, cytochrome P-450scc, or cytochrome c to AEDANS-adrenodoxin was found to quench the fluorescence of the AEDANS in a manner consistent with the formation of 1:1 binary complexes. 1,5-AEDANS ferredoxin reductase Bos taurus
2 Forster energy transfer calculations indicated that the AEDANS label on adrenodoxin was 42 A from the heme group in cytochrome c, 36 A from the FAD group in adrenodoxin reductase, and 58 A from the heme group in cytochrome P-450scc in the respective binary complexes. 1,5-AEDANS ferredoxin reductase Bos taurus