Pub. Date : 2018 May 21
PMID : 29883401
4 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | For this purpose we present a short biotinylated peptide derivative with a chloromethyl ketone group, biotin-RQRR-CMK, as an activity-based probe for matriptase-2. | Biotin | transmembrane serine protease 6 | Homo sapiens |
| 2 | Biotin-RQRR-CMK was kinetically characterized and exhibited a second-order rate constant of inactivation (kinac/Ki) of 10,800 M−1 s−1 towards the matriptase-2 activity in the supernatant of transfected human embryonic kidney (HEK) cells. | Biotin | transmembrane serine protease 6 | Homo sapiens |
| 3 | Biotin-RQRR-CMK was able to label active matriptase-2, as visualized in western blot experiments. | Biotin | transmembrane serine protease 6 | Homo sapiens |
| 4 | Pretreatment with aprotinin, an active-site directed inhibitor of serine proteases, protected matriptase-2 from the reaction with biotin-RQRR-CMK. | Biotin | transmembrane serine protease 6 | Homo sapiens |