Title : Impact of Site-Specific Conjugation of ScFv to Multifunctional Nanomedicines Using Second Generation Maleimide.

Pub. Date : 2018 May 16

PMID : 29553717






2 Functional Relationships(s)
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1 We developed two novel bioengineered scFv carrying two cysteines located (i) at the end (4D5.1-cys2) or (ii) at the beginning (4D5.2-cys2) of its hexahistidine tag. Cysteine immunglobulin heavy chain variable region Homo sapiens
2 Our data indicated that the insertion of cysteines at the beginning of the hexahistidine tag was allowed to obtain nearly 2-fold conjugation efficiency (13 scFv/NP) compared to NMs using classical maleimide. Cysteine immunglobulin heavy chain variable region Homo sapiens