Title : Time-resolved monitoring of enzyme activity with ultrafast Hyper-CEST spectroscopy.

Pub. Date : 2018 Jul

PMID : 29274298






1 Functional Relationships(s)
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1 This allows monitoring the dynamic progress of the conversion of lysine to cadaverine with a temporal resolution of ~30 s. Moreover, the method only requires to sample the very early onset of the reaction (<0.5% of substrate conversion where the host itself is required only at muM concentrations) at comparatively low reaction rates, thus saving enzyme material and reducing NMR acquisition time. Cadaverine latexin Homo sapiens