Title : Recombinant alpha-fetoprotein receptor-binding domain co-expression with polyglutamate tags facilitates in vivo folding in E. coli.

Pub. Date : 2018 Mar

PMID : 29127003






1 Functional Relationships(s)
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1 In this study we evaluated the minimal quantity of high density negatively charged EEEEVE amino acid repeats (pGlu) necessary to switch the recombinant receptor-binding domain of human alpha-fetoprotein (rbdAFP) expression almost entirely from the inclusion bodies to the soluble cytoplasmic fraction in E. coli. Pyrrolidonecarboxylic Acid alpha fetoprotein Homo sapiens