Title : HEK293T cell lines defective for O-linked glycosylation.

Pub. Date : 2017

PMID : 28654657






3 Functional Relationships(s)
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1 Deficient glycosylation in the GALE knockout cell line could be rescued by the addition of galactose and N-acetylgalactosamine (GalNAc) to the cell culture media. Galactose UDP-galactose-4-epimerase Homo sapiens
2 However, when key enzymes of the galactose/GalNAc salvage pathways were disrupted in tandem (GALE+GALK1 or GALE+GALK2), O-glycosylation was eliminated and could not be rescued by the addition of either galactose plus GalNAc or UDP-galactose plus UDP-GalNAc. Galactose UDP-galactose-4-epimerase Homo sapiens
3 However, when key enzymes of the galactose/GalNAc salvage pathways were disrupted in tandem (GALE+GALK1 or GALE+GALK2), O-glycosylation was eliminated and could not be rescued by the addition of either galactose plus GalNAc or UDP-galactose plus UDP-GalNAc. Galactose UDP-galactose-4-epimerase Homo sapiens