Pub. Date : 1985 Jan-Feb
PMID : 2858378
9 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Loss of rat liver microsomal cytochrome P-450 during methimazole metabolism. | Methimazole | cytochrome P450, family 2, subfamily g, polypeptide 1 | Rattus norvegicus |
| 2 | The role of flavin-containing monooxygenase (FMO) in the decrease in cytochrome P-450 content during the microsomal metabolism of methimazole (N-methyl-2-mercaptoimidazole) was investigated by heat inactivation of FMO. | Methimazole | cytochrome P450, family 2, subfamily g, polypeptide 1 | Rattus norvegicus |
| 3 | The role of flavin-containing monooxygenase (FMO) in the decrease in cytochrome P-450 content during the microsomal metabolism of methimazole (N-methyl-2-mercaptoimidazole) was investigated by heat inactivation of FMO. | Methimazole | cytochrome P450, family 2, subfamily g, polypeptide 1 | Rattus norvegicus |
| 4 | Incubation of liver microsomes from untreated Fischer 344 rats with NADPH and methimazole resulted in a 25% loss of cytochrome P-450 detectable as its ferrous-carbon monoxide complex. | Methimazole | cytochrome P450, family 2, subfamily g, polypeptide 1 | Rattus norvegicus |
| 5 | The same extent of cytochrome P-450 loss was observed with 1 and 20 mM methimazole, suggesting saturation of the process. | Methimazole | cytochrome P450, family 2, subfamily g, polypeptide 1 | Rattus norvegicus |
| 6 | These results indicate that FMO-catalyzed metabolism of methimazole is necessary for the loss of cytochrome P-450 in microsomes from untreated rats. | Methimazole | cytochrome P450, family 2, subfamily g, polypeptide 1 | Rattus norvegicus |
| 7 | There was no loss of cytochrome P-450 when glutathione was included in the incubation with methimazole, suggesting that cytochrome P-450 loss was due to an interaction with oxygenated metabolites of methimazole formed by FMO. | Methimazole | cytochrome P450, family 2, subfamily g, polypeptide 1 | Rattus norvegicus |
| 8 | Losses of cytochrome P-450 were also observed after incubation of microsomes from phenobarbital- (31%) of beta-naphthoflavone-pretreated rats (44%) with NADPH and methimazole. | Methimazole | cytochrome P450, family 2, subfamily g, polypeptide 1 | Rattus norvegicus |
| 9 | These results indicate that both phenobarbital and beta-naphthoflavone induce isozymes of cytochrome P-450 capable of directly activating methimazole. | Methimazole | cytochrome P450, family 2, subfamily g, polypeptide 1 | Rattus norvegicus |