Title : 3-Hydroxy-3-methylglutaryl coenzyme A reductase: regulation of enzymatic activity by phosphorylation and dephosphorylation.

Pub. Date : 1978 Aug

PMID : 278983






4 Functional Relationships(s)
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1 Incubation of microsomal HMG-CoA reductase with [gamma-32P]ATP+Mg2+ was associated with a reciprocal increase in [32P]protein-bound radioactivity and a decrease in enzymatic activity. Phosphorus-32 3-hydroxy-3-methylglutaryl-CoA reductase Homo sapiens
2 Incubation of 32P-labeled microsomal HMG-CoA reductase with a partially purified cytosolic phosphatase resulted in a time-dependent reciprocal release of [32P]protein-bound radioactivity and reactivation of enzyme activity. Phosphorus-32 3-hydroxy-3-methylglutaryl-CoA reductase Homo sapiens
3 Incubation of 32P-labeled microsomal HMG-CoA reductase with a partially purified cytosolic phosphatase resulted in a time-dependent reciprocal release of [32P]protein-bound radioactivity and reactivation of enzyme activity. Phosphorus-32 3-hydroxy-3-methylglutaryl-CoA reductase Homo sapiens
4 Sodium dodecyl sulfate electrophoresis of the [gamma-32P]immunoprecipitate revealed that the 32P radioactivity was located in the electrophoretic position of HMG-CoA reductase. Phosphorus-32 3-hydroxy-3-methylglutaryl-CoA reductase Homo sapiens