Title : Interactions with hemoglobin: a source of error in measurements of transketolase activity in hemolysates.

Pub. Date : 1989 Apr 14

PMID : 2743579






2 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 Measurements of the activity of transketolase in human erythrocyte lysates by an assay coupled to NADH oxidation indicate that interactions of assay substrates with hemoglobin can give rise to overestimations of transketolase activity. NAD transketolase Homo sapiens
2 Thus, in lysates containing methemoglobin, NADH oxidation can be due firstly to methemoglobin reductase activity or secondly to the monooxygenase activity of methemoglobin, for which the substrate can be ribose 5-phosphate, a substrate also of transketolase. NAD transketolase Homo sapiens