Title : Recognition and binding of β-lactam antibiotics to bovine serum albumin by frontal affinity chromatography in combination with spectroscopy and molecular docking.

Pub. Date : 2016 Mar 1

PMID : 26882128






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1 This work was designed to investigate the recognition and binding of three typical beta-lactam antibiotics including penicillin G (Pen G), penicillin V (Pen V) and cefalexin (Cef) with bovine serum albumin (BSA) by frontal affinity chromatography in combination with UV-vis absorption spectra, fluorescence emission spectra, binding site marker competitive experiment and molecular docking under simulated physiological conditions. Penicillin G proprotein convertase subtilisin/kexin type 1 inhibitor Homo sapiens