Title : Effective isotope labeling of proteins in a mammalian expression system.

Pub. Date : 2015

PMID : 26577736






1 Functional Relationships(s)
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1 We describe a roadmap to produce isotopically labeled (15)N and (13)C posttranslationally modified proteins, such as the outer domain of HIV-1 gp120, which has four disulfide bonds and 15 potential sites of N-linked glycosylation. Disulfides Envelope surface glycoprotein gp160, precursor Human immunodeficiency virus 1