Title : New supercharging reagents produce highly charged protein ions in native mass spectrometry.

Pub. Date : 2015 Nov 7

PMID : 26421324






1 Functional Relationships(s)
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1 In aqueous solution, 2-thiophenone and 4-hydroxymethyl-1,3-dioxolan-2-one (HD) at a concentration of 2% by volume can increase the average charge of cytochrome c and myoglobin by up to 163%, resulting in even higher charge states than those that are produced from water/methanol/acid solutions in which these proteins are denatured. Water cytochrome c, somatic Homo sapiens