Pub. Date : 2015 Oct
PMID : 26179622
5 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Overexpression of ZWF1 and POS5 improves carotenoid biosynthesis in recombinant Saccharomyces cerevisiae. | Carotenoids | NADH kinase | Saccharomyces cerevisiae S288C |
| 2 | NADPH is a key cofactor for carotenoid biosynthesis, while glucose-6-phosphate dehydrogenase (Zwf1) and an NADH kinase (Pos5) are the two main NADPH-supplying sources in S. cerevisiae. | Carotenoids | NADH kinase | Saccharomyces cerevisiae S288C |
| 3 | Here, the effect of ZWF1 and POS5 overexpression on carotenoid yield in recombinant S. cerevisiae was explored. | Carotenoids | NADH kinase | Saccharomyces cerevisiae S288C |
| 4 | Therefore, improvement of NADPH supply contributed to carotenoids biosynthesis in S. cerevisiae and overexpression of POS5 was more effective than overexpression of ZWF1. | Carotenoids | NADH kinase | Saccharomyces cerevisiae S288C |
| 5 | When ZWF1 and POS5 were overexpressed in a carotenoid-producing S. cerevisiae strain individually, the total yield of lycopene and beta-carotene increased by 59 9% and 81 4%, respectively, and the final product beta-carotene yield increased by 18 8% and 65 6% respectively. | Carotenoids | NADH kinase | Saccharomyces cerevisiae S288C |