Title : Comparison of immunoglobulin binding capacities and ligand leakage using eight different protein A affinity chromatography matrices.

Pub. Date : 1989 Nov 30

PMID : 2600425






1 Functional Relationships(s)
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1 Depending on the matrix used, the IgG3 eluted from the protein A columns exhibited a contamination of 1.8-88 ppm (weight/weight) protein A. Amongst the gels with IgG3 capacities greater than 10 mg/ml, the least contamination with protein A was observed in the IgG3 fractions from immobilized rProtein A and Protein A-Sepharose CL-4B (Fermentech). Sepharose CL 4B immunoglobulin heavy constant gamma 3 (G3m marker) Homo sapiens