Pub. Date : 2015 Jan
PMID : 25877313
12 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | OBJECTIVE: To explore the changes of anticancer efficiency of 5"-deoxy-5-fluorouridine (5"-DFUR) and 5-fluorouracil (5-Fu) in colorectal cancer cell line HT29 and LS174T cells after transfection of thymidine phosphorylase (TP) cDNA with a lentiviral vector. | Fluorouracil | thymidine phosphorylase | Homo sapiens |
| 2 | OBJECTIVE: To explore the changes of anticancer efficiency of 5"-deoxy-5-fluorouridine (5"-DFUR) and 5-fluorouracil (5-Fu) in colorectal cancer cell line HT29 and LS174T cells after transfection of thymidine phosphorylase (TP) cDNA with a lentiviral vector. | Fluorouracil | thymidine phosphorylase | Homo sapiens |
| 3 | Finally, the concentration of converted 5-Fu was detected by high performance liquid chromatography (HPLC) either in the medium containing a series of concentrations of 5"-DFUR, in which HT29/HT29-TP or LS174T/LS174T-TP cells were cultured, or in the cell culture lysates. | Fluorouracil | thymidine phosphorylase | Homo sapiens |
| 4 | Finally, the concentration of converted 5-Fu was detected by high performance liquid chromatography (HPLC) either in the medium containing a series of concentrations of 5"-DFUR, in which HT29/HT29-TP or LS174T/LS174T-TP cells were cultured, or in the cell culture lysates. | Fluorouracil | thymidine phosphorylase | Homo sapiens |
| 5 | The IC50 values of 5-Fu of HT29-TP cells and its parents cells were (5.42 +- 0.75) micromol/L and (14.19 +- 0.97) micromol/L, respectively (P < 0.05), while (4.41 +- 0.96)micromol/L in LS174T-TP cells and (16.42 +- 2.12)micromol/L in its parents cells, respectively (P < 0.05). | Fluorouracil | thymidine phosphorylase | Homo sapiens |
| 6 | The IC50 values of 5-Fu of HT29-TP cells and its parents cells were (5.42 +- 0.75) micromol/L and (14.19 +- 0.97) micromol/L, respectively (P < 0.05), while (4.41 +- 0.96)micromol/L in LS174T-TP cells and (16.42 +- 2.12)micromol/L in its parents cells, respectively (P < 0.05). | Fluorouracil | thymidine phosphorylase | Homo sapiens |
| 7 | The HPLC results showed that the 5-Fu concentration detected from media contained a series of concentrations of 5"-DFUR for culturing HT29-TP and LS174T-TP cells were 12.2 to 28.7-folds and 13.1 to 23.6-folds, respectively, higher than that in their parents cells, (P < 0.01 for all). | Fluorouracil | thymidine phosphorylase | Homo sapiens |
| 8 | The HPLC results showed that the 5-Fu concentration detected from media contained a series of concentrations of 5"-DFUR for culturing HT29-TP and LS174T-TP cells were 12.2 to 28.7-folds and 13.1 to 23.6-folds, respectively, higher than that in their parents cells, (P < 0.01 for all). | Fluorouracil | thymidine phosphorylase | Homo sapiens |
| 9 | Otherwise, just a little of 5-Fu was detected in the two TP-transfected cells lysate, about 0.9% to 4.2% of 5-Fu detected in the media of the same cultured cells, whereas no 5-Fu was detected in the two parent cell lysates. | Fluorouracil | thymidine phosphorylase | Homo sapiens |
| 10 | CONCLUSIONS: Transfection of TP cDNA into colorectal cancer cell lines HT29 and LS174T with lentiviral vector can improve the expression of both TP mRNA and TP protein levels in passaged cells, enhance the conversion of 5-Fu from 5"-DFUR in the medium, and result in an enhanced anticancer effect on these two cell lines. | Fluorouracil | thymidine phosphorylase | Homo sapiens |
| 11 | CONCLUSIONS: Transfection of TP cDNA into colorectal cancer cell lines HT29 and LS174T with lentiviral vector can improve the expression of both TP mRNA and TP protein levels in passaged cells, enhance the conversion of 5-Fu from 5"-DFUR in the medium, and result in an enhanced anticancer effect on these two cell lines. | Fluorouracil | thymidine phosphorylase | Homo sapiens |
| 12 | CONCLUSIONS: Transfection of TP cDNA into colorectal cancer cell lines HT29 and LS174T with lentiviral vector can improve the expression of both TP mRNA and TP protein levels in passaged cells, enhance the conversion of 5-Fu from 5"-DFUR in the medium, and result in an enhanced anticancer effect on these two cell lines. | Fluorouracil | thymidine phosphorylase | Homo sapiens |