Title : Reporter-based growth assay for systematic analysis of protein degradation.

Pub. Date : 2014 Nov 6

PMID : 25406949






3 Functional Relationships(s)
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1 The method is based on an established selection system where uracil auxotrophy of URA3-deleted yeast cells is rescued by an exogenously expressed reporter protein, comprised of a fusion between the essential URA3 gene and a degradation determinant (degron). Uracil orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C
2 The method is based on an established selection system where uracil auxotrophy of URA3-deleted yeast cells is rescued by an exogenously expressed reporter protein, comprised of a fusion between the essential URA3 gene and a degradation determinant (degron). Uracil orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C
3 As cell growth in uracil-deficient medium is proportional to the relative levels of Ura3, growth kinetics are entirely dependent on the reporter protein degradation. Uracil orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C