Pub. Date : 2014 Nov 6
PMID : 25406949
3 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
1 | The method is based on an established selection system where uracil auxotrophy of URA3-deleted yeast cells is rescued by an exogenously expressed reporter protein, comprised of a fusion between the essential URA3 gene and a degradation determinant (degron). | Uracil | orotidine-5'-phosphate decarboxylase | Saccharomyces cerevisiae S288C |
2 | The method is based on an established selection system where uracil auxotrophy of URA3-deleted yeast cells is rescued by an exogenously expressed reporter protein, comprised of a fusion between the essential URA3 gene and a degradation determinant (degron). | Uracil | orotidine-5'-phosphate decarboxylase | Saccharomyces cerevisiae S288C |
3 | As cell growth in uracil-deficient medium is proportional to the relative levels of Ura3, growth kinetics are entirely dependent on the reporter protein degradation. | Uracil | orotidine-5'-phosphate decarboxylase | Saccharomyces cerevisiae S288C |