Pub. Date : 1989 Feb 25
PMID : 2492530
8 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | A new water-soluble color reagent, 4-N,N-dimethylaminoazobenzene-4"-isothiocyano-2"-sulfonic acid (S-DABITC), was used to identify lysine residues of antithrombin III which participate in the binding of heparin. | 4-(N,N-dimethylaminoazobenzene)-4'-isothiocyanate | serpin family C member 1 | Homo sapiens |
| 2 | Antithrombin, modified with S-DABITC in the presence and absence of low molecular weight heparin (Mr 5000) was reduced, carboxymethylated, and digested with trypsin. | 4-(N,N-dimethylaminoazobenzene)-4'-isothiocyanate | serpin family C member 1 | Homo sapiens |
| 3 | When antithrombin was preincubated with heparin (2-fold by weight), followed by S-DABITC modification, the recovery of peptide T4 remained unchanged, but the recoveries of T1, T2, and T3 were reduced by 93, 86, and 98%, respectively. | 4-(N,N-dimethylaminoazobenzene)-4'-isothiocyanate | serpin family C member 1 | Homo sapiens |
| 4 | Thus, binding of heparin to human antithrombin diminished S-DABITC modification at Lys-107, Lys-125, and Lys-136, but at the same time enhanced S-DABITC modification at Lys-236. | 4-(N,N-dimethylaminoazobenzene)-4'-isothiocyanate | serpin family C member 1 | Homo sapiens |
| 5 | Thus, binding of heparin to human antithrombin diminished S-DABITC modification at Lys-107, Lys-125, and Lys-136, but at the same time enhanced S-DABITC modification at Lys-236. | 4-(N,N-dimethylaminoazobenzene)-4'-isothiocyanate | serpin family C member 1 | Homo sapiens |
| 6 | We conclude that Lys-107, Lys-125, and Lys-136 are situated within the heparin-binding site of human antithrombin and that binding of heparin to antithrombin causes a conformational change of antithrombin that leads to the exposure of Lys-236 for S-DABITC modification. | 4-(N,N-dimethylaminoazobenzene)-4'-isothiocyanate | serpin family C member 1 | Homo sapiens |
| 7 | We conclude that Lys-107, Lys-125, and Lys-136 are situated within the heparin-binding site of human antithrombin and that binding of heparin to antithrombin causes a conformational change of antithrombin that leads to the exposure of Lys-236 for S-DABITC modification. | 4-(N,N-dimethylaminoazobenzene)-4'-isothiocyanate | serpin family C member 1 | Homo sapiens |
| 8 | We conclude that Lys-107, Lys-125, and Lys-136 are situated within the heparin-binding site of human antithrombin and that binding of heparin to antithrombin causes a conformational change of antithrombin that leads to the exposure of Lys-236 for S-DABITC modification. | 4-(N,N-dimethylaminoazobenzene)-4'-isothiocyanate | serpin family C member 1 | Homo sapiens |