Title : Highly efficient N-glycoproteomic sample preparation by combining C(18) and graphitized carbon adsorbents.

Pub. Date : 2014 May

PMID : 24668066






4 Functional Relationships(s)
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1 Conventional N-glycoproteome analysis usually applies C18 reversed-phase (RP) adsorbent for sample purification, which will lead to unavoidable sample loss due to the high hydrophilicity of N-glycopeptides. n-glycopeptides Bardet-Biedl syndrome 9 Homo sapiens
2 It was observed that the small hydrophilic N-glycopeptides that cannot retain onto C18 adsorbent can be captured by the graphitized carbon, while the large hydrophobic N-glycopeptides that cannot retain onto the graphitized carbon can be feasibly captured by the C18 adsorbent. n-glycopeptides Bardet-Biedl syndrome 9 Homo sapiens
3 Comparing with sample purification by using C18 adsorbent only, 28.5 % more N-glycopeptides were identified by combining both C18 and PGC adsorbents. n-glycopeptides Bardet-Biedl syndrome 9 Homo sapiens
4 Comparing with sample purification by using C18 adsorbent only, 28.5 % more N-glycopeptides were identified by combining both C18 and PGC adsorbents. n-glycopeptides Bardet-Biedl syndrome 9 Homo sapiens