Title : Production of recombinant Chikungunya virus envelope 2 protein in Escherichia coli.

Pub. Date : 2014 Mar

PMID : 24337252






1 Functional Relationships(s)
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1 Expression of rCHIKV E2 protein in E. coli was induced with 1 mM isopropyl-beta-thiogalactoside (IPTG) at ~23 g dry cell weight (DCW) per liter of culture and yielded an insoluble protein aggregating to form inclusion bodies. Isopropyl Thiogalactoside ubiquitin conjugating enzyme E2 B Homo sapiens