Pub. Date : 2013
PMID : 23603888
3 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
1 | Using a newly developed near-infrared (NIR) dye that fluoresces at two different wavelengths (dichromic fluorescence, DCF), we discovered a new fluorescent substrate for Akt, also known as protein kinase B, and a method to quantitatively report this enzyme"s activity in real time. | Pentostatin | AKT serine/threonine kinase 1 | Homo sapiens |
2 | Upon insulin activation of cellular Akt, the enzyme multi-phosphorylated a single serine residue of a diserine DCF substrate in a time-dependent manner, culminating in monophospho- to triphospho-serine products. | Pentostatin | AKT serine/threonine kinase 1 | Homo sapiens |
3 | The DCF mechanism provides unparalleled potential to assess the stimulation, sustainability, and reversibility of Akt activation longitudinally. | Pentostatin | AKT serine/threonine kinase 1 | Homo sapiens |