Title : Dual fluorescent molecular substrates selectively report the activation, sustainability and reversibility of cellular PKB/Akt activity.

Pub. Date : 2013

PMID : 23603888






3 Functional Relationships(s)
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1 Using a newly developed near-infrared (NIR) dye that fluoresces at two different wavelengths (dichromic fluorescence, DCF), we discovered a new fluorescent substrate for Akt, also known as protein kinase B, and a method to quantitatively report this enzyme"s activity in real time. Pentostatin AKT serine/threonine kinase 1 Homo sapiens
2 Upon insulin activation of cellular Akt, the enzyme multi-phosphorylated a single serine residue of a diserine DCF substrate in a time-dependent manner, culminating in monophospho- to triphospho-serine products. Pentostatin AKT serine/threonine kinase 1 Homo sapiens
3 The DCF mechanism provides unparalleled potential to assess the stimulation, sustainability, and reversibility of Akt activation longitudinally. Pentostatin AKT serine/threonine kinase 1 Homo sapiens