Pub. Date : 2013 Jan
PMID : 23600203
8 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | [Comparison of the effect between procaine and 5-aza-dc on wnt inhibitory factor 1 promoter methylation of HepG2]. | Procaine | WNT inhibitory factor 1 | Homo sapiens |
| 2 | OBJECTIVE: To observe the CpG island methylation status, mRNA and protein expression of the Wnt inhibitory factor-1 (Wif-1) gene with procaine or 5"-Aza-2"-deoxycycytidine (5-aza-dc) on HepG2. | Procaine | WNT inhibitory factor 1 | Homo sapiens |
| 3 | OBJECTIVE: To observe the CpG island methylation status, mRNA and protein expression of the Wnt inhibitory factor-1 (Wif-1) gene with procaine or 5"-Aza-2"-deoxycycytidine (5-aza-dc) on HepG2. | Procaine | WNT inhibitory factor 1 | Homo sapiens |
| 4 | RESULTS: (1) RT-PCR and Western blot exhibited that both Wif-1 mRNA and Wif-1 protein expression were positive in groups treated with procaine and 5-aza-dc and highly positive in the L0 cells group, but weakly even negative in the HepG2 cells without any treatment, the difference were statistically significant (P < 0.05) (procaine experimental group was higher than that of 5-aza-dc experimental group, P < 0.05). | Procaine | WNT inhibitory factor 1 | Homo sapiens |
| 5 | RESULTS: (1) RT-PCR and Western blot exhibited that both Wif-1 mRNA and Wif-1 protein expression were positive in groups treated with procaine and 5-aza-dc and highly positive in the L0 cells group, but weakly even negative in the HepG2 cells without any treatment, the difference were statistically significant (P < 0.05) (procaine experimental group was higher than that of 5-aza-dc experimental group, P < 0.05). | Procaine | WNT inhibitory factor 1 | Homo sapiens |
| 6 | RESULTS: (1) RT-PCR and Western blot exhibited that both Wif-1 mRNA and Wif-1 protein expression were positive in groups treated with procaine and 5-aza-dc and highly positive in the L0 cells group, but weakly even negative in the HepG2 cells without any treatment, the difference were statistically significant (P < 0.05) (procaine experimental group was higher than that of 5-aza-dc experimental group, P < 0.05). | Procaine | WNT inhibitory factor 1 | Homo sapiens |
| 7 | RESULTS: (1) RT-PCR and Western blot exhibited that both Wif-1 mRNA and Wif-1 protein expression were positive in groups treated with procaine and 5-aza-dc and highly positive in the L0 cells group, but weakly even negative in the HepG2 cells without any treatment, the difference were statistically significant (P < 0.05) (procaine experimental group was higher than that of 5-aza-dc experimental group, P < 0.05). | Procaine | WNT inhibitory factor 1 | Homo sapiens |
| 8 | CONCLUSION: Both of procaine and 5-aza-dc can reverse the abnormal methylation of Wif-1 gene. | Procaine | WNT inhibitory factor 1 | Homo sapiens |