Title : [Cloning and prokaryotic expression of human secreted IL-16 gene].

Pub. Date : 2012 Dec

PMID : 23232511






2 Functional Relationships(s)
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1 The expression of secreted hIL-16 was induced with IPTG and identified by SDS-PAGE and Western blotting. Sodium Dodecyl Sulfate interleukin 16 Homo sapiens
2 The prokaryotic expression vector pMAL-IL-16 we constructed was successfully transformed into E.coli DH5alpha, and under the induction of IPTG, we found the expression of the recombinant fusion protein with relative molecular weight (M(r);) being 56 000 as expected and confirmed by SDS-PAGE and Western blotting. Sodium Dodecyl Sulfate interleukin 16 Homo sapiens