Title : Production of bioactive flavonol rhamnosides by expression of plant genes in Escherichia coli.

Pub. Date : 2012 Nov 7

PMID : 23072384






4 Functional Relationships(s)
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Protein Name
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1 To synthesize flavonoid rhamnoside, a strain of E. coli expressing UDP-rhamnose flavonol glycosyltransferase (AtUGT78D1) from Arabidopsis thaliana was used to produce quercetin 3-O-rhamnoside. quercitrin UDP-glucosyl transferase 78D1 Arabidopsis thaliana
2 A strain of E. coli rfbD (encoding dTDP-4-dehydrorhamnose reductase) expressing AtUGT78D1, which is involved in the final step of thymidine diphosphate rhamnose (TDP-rhamnose) biosynthesis, did not produce quercetin 3-O-rhamnoside, meaning that AtUGT78D1 used endogenous TDP-rhamnose as a sugar donor to produce quercetin 3-O-rhamnoside. quercitrin UDP-glucosyl transferase 78D1 Arabidopsis thaliana
3 A strain of E. coli rfbD (encoding dTDP-4-dehydrorhamnose reductase) expressing AtUGT78D1, which is involved in the final step of thymidine diphosphate rhamnose (TDP-rhamnose) biosynthesis, did not produce quercetin 3-O-rhamnoside, meaning that AtUGT78D1 used endogenous TDP-rhamnose as a sugar donor to produce quercetin 3-O-rhamnoside. quercitrin UDP-glucosyl transferase 78D1 Arabidopsis thaliana
4 The production of quercetin 3-O-rhamnoside could be increased by up to 160% by co-expressing AtUGT78D1 and rhamnose synthase gene 2 (RHM2), which catalyzes the conversion of UDP-glucose into UDP-rhamnose. quercitrin UDP-glucosyl transferase 78D1 Arabidopsis thaliana