Title : An N-glycosylation Analysis of Human Alpha-2-Macroglobulin Using an Integrated Approach.

Pub. Date : 2012 May 31

PMID : 23028207






2 Functional Relationships(s)
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1 Herein, the detailed N-glycosylation pattern of human serum alpha-2-macroglobulin was studied using an integrative approach, including permethylation of N-glycans, collision induced dissociation (CID) and electron transfer dissociation (ETD) of chymotryptic N-glycopeptides, and partial deglycosylation of chymotryptic N-glycopeptides with endo-beta-N-acetylglucosaminidase F3 (Endo F3). n-glycopeptides alpha-2-macroglobulin Homo sapiens
2 Herein, the detailed N-glycosylation pattern of human serum alpha-2-macroglobulin was studied using an integrative approach, including permethylation of N-glycans, collision induced dissociation (CID) and electron transfer dissociation (ETD) of chymotryptic N-glycopeptides, and partial deglycosylation of chymotryptic N-glycopeptides with endo-beta-N-acetylglucosaminidase F3 (Endo F3). n-glycopeptides alpha-2-macroglobulin Homo sapiens