Title : Quantitative kinetics of proteolytic enzymes determined by a surface concentration-based assay using peptide arrays.

Pub. Date : 2012 Aug 21

PMID : 22768390






1 Functional Relationships(s)
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1 For fabrication of the peptide arrays, substrate peptides of cMMP-3, caspase-3, caspase-9, and calpain-1 were functionalized with TAMRA and cysteine, and were immobilized onto amine-functionalized arrays using a heterobifunctional linker, N-[gamma-maleimidobutyloxy]succinimide ester. Cysteine caspase 3 Homo sapiens