Pub. Date : 2012 Jul
PMID : 22231145
7 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | The aim of this study was to examine the mechanisms underlying interleukin-1beta (IL-1beta)-induced cytosolic phospholipase A2 (cPLA2) expression through ROS generation as modulated by HO-1 in RA synovial fibroblasts (RASFs). | Reactive Oxygen Species | interleukin 1 beta | Mus musculus |
| 2 | The aim of this study was to examine the mechanisms underlying interleukin-1beta (IL-1beta)-induced cytosolic phospholipase A2 (cPLA2) expression through ROS generation as modulated by HO-1 in RA synovial fibroblasts (RASFs). | Reactive Oxygen Species | interleukin 1 beta | Mus musculus |
| 3 | METHODS: IL-1beta-induced ROS generation was determined by flow cytometry. | Reactive Oxygen Species | interleukin 1 beta | Mus musculus |
| 4 | The involvement of MAPKs and NADPH oxidase (NOX)/ROS in IL-1beta-induced cPLA2 expression was investigated using pharmacologic inhibitors and transfection with small interfering RNAs (siRNAs) and was analyzed by Western blotting and promoter assay. | Reactive Oxygen Species | interleukin 1 beta | Mus musculus |
| 5 | RESULTS: IL-1beta-induced cPLA2 expression was mediated through NOX activation/ROS production, which was attenuated by N-acetylcysteine (NAC; a scavenger of ROS), the inhibitors of NOX (diphenyleneiodonium chloride and apocynin), MEK-1/2 (U0126), and JNK-1/2 (SP600125), transfection with the respective siRNAs, and the overexpression of HO-1 in RASFs. | Reactive Oxygen Species | interleukin 1 beta | Mus musculus |
| 6 | RESULTS: IL-1beta-induced cPLA2 expression was mediated through NOX activation/ROS production, which was attenuated by N-acetylcysteine (NAC; a scavenger of ROS), the inhibitors of NOX (diphenyleneiodonium chloride and apocynin), MEK-1/2 (U0126), and JNK-1/2 (SP600125), transfection with the respective siRNAs, and the overexpression of HO-1 in RASFs. | Reactive Oxygen Species | interleukin 1 beta | Mus musculus |
| 7 | CONCLUSION: In RASFs, IL-1beta induced cPLA2 expression via activation of p42/p44 MAPK and JNK-1/2, leading to p47phox phosphorylation, ROS production, and AP-1 activation. | Reactive Oxygen Species | interleukin 1 beta | Mus musculus |