Pub. Date : 2012 Jan 6
PMID : 22166205
8 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Using the demethylating drug 5-aza-2"-deoxycytidine (5-Aza), we tested whether and how BACE1 expression is regulated in mouse BV-2 microglial cells. | Decitabine | beta-site APP cleaving enzyme 1 | Mus musculus |
| 2 | 5-Aza increased both BACE1 mRNA and protein levels in a dose-dependent manner. | Decitabine | beta-site APP cleaving enzyme 1 | Mus musculus |
| 3 | Bisulfite-sequencing analysis revealed that two CpG sites at positions +298 and +351 in the 5"-untranslated region (5"-UTR) of the BACE1 gene were specifically demethylated in BV-2 cells treated with 5-Aza. | Decitabine | beta-site APP cleaving enzyme 1 | Mus musculus |
| 4 | To assess whether these two CpG sites play an important role in 5-Aza-induced transcriptional activation of BACE1, we constructed a BACE1 gene promoter including the 5"-UTR (-1136 to +500) fused to a CpG-free luciferase gene (pCpGL-BACE1) and its mutant pCpGL-BACE1-AA, which has substituted CG dinucleotides at the two CpG sites of pCpGL-BACE1 to AA. | Decitabine | beta-site APP cleaving enzyme 1 | Mus musculus |
| 5 | To assess whether these two CpG sites play an important role in 5-Aza-induced transcriptional activation of BACE1, we constructed a BACE1 gene promoter including the 5"-UTR (-1136 to +500) fused to a CpG-free luciferase gene (pCpGL-BACE1) and its mutant pCpGL-BACE1-AA, which has substituted CG dinucleotides at the two CpG sites of pCpGL-BACE1 to AA. | Decitabine | beta-site APP cleaving enzyme 1 | Mus musculus |
| 6 | To assess whether these two CpG sites play an important role in 5-Aza-induced transcriptional activation of BACE1, we constructed a BACE1 gene promoter including the 5"-UTR (-1136 to +500) fused to a CpG-free luciferase gene (pCpGL-BACE1) and its mutant pCpGL-BACE1-AA, which has substituted CG dinucleotides at the two CpG sites of pCpGL-BACE1 to AA. | Decitabine | beta-site APP cleaving enzyme 1 | Mus musculus |
| 7 | To assess whether these two CpG sites play an important role in 5-Aza-induced transcriptional activation of BACE1, we constructed a BACE1 gene promoter including the 5"-UTR (-1136 to +500) fused to a CpG-free luciferase gene (pCpGL-BACE1) and its mutant pCpGL-BACE1-AA, which has substituted CG dinucleotides at the two CpG sites of pCpGL-BACE1 to AA. | Decitabine | beta-site APP cleaving enzyme 1 | Mus musculus |
| 8 | To assess whether these two CpG sites play an important role in 5-Aza-induced transcriptional activation of BACE1, we constructed a BACE1 gene promoter including the 5"-UTR (-1136 to +500) fused to a CpG-free luciferase gene (pCpGL-BACE1) and its mutant pCpGL-BACE1-AA, which has substituted CG dinucleotides at the two CpG sites of pCpGL-BACE1 to AA. | Decitabine | beta-site APP cleaving enzyme 1 | Mus musculus |