Pub. Date : 2012 May
PMID : 22160573
9 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | In this work, fluorescence spectroscopy in combination with circular dichroism spectroscopy and molecular modeling was employed to investigate the binding of 10-hydroxycamptothecin (HCPT) to human serum albumin (HSA) under simulative physiological conditions. | 10-hydroxycamptothecin | albumin | Homo sapiens |
| 2 | In this work, fluorescence spectroscopy in combination with circular dichroism spectroscopy and molecular modeling was employed to investigate the binding of 10-hydroxycamptothecin (HCPT) to human serum albumin (HSA) under simulative physiological conditions. | 10-hydroxycamptothecin | albumin | Homo sapiens |
| 3 | The experiment results showed that the fluorescence quenching of HSA by HCPT was a result of the formation of HCPT-HSA complex. | 10-hydroxycamptothecin | albumin | Homo sapiens |
| 4 | The experiment results showed that the fluorescence quenching of HSA by HCPT was a result of the formation of HCPT-HSA complex. | 10-hydroxycamptothecin | albumin | Homo sapiens |
| 5 | The corresponding association constants (K (a)) between HCPT and HSA at four different temperatures were determined according to the modified Stern-Volmer equation. | 10-hydroxycamptothecin | albumin | Homo sapiens |
| 6 | The results of thermodynamic parameters DeltaG, DeltaH, and DeltaS indicated that hydrogen bonds and van der Waals forces played major roles for HCPT-HSA association. | 10-hydroxycamptothecin | albumin | Homo sapiens |
| 7 | Site marker competitive displacement experiment indicated that the binding of HCPT to HSA primarily took place in sub-domain IIA (site I). | 10-hydroxycamptothecin | albumin | Homo sapiens |
| 8 | The conformational investigation showed that the presence of HCPT decreased the alpha-helical content of HSA and induced the slight unfolding of the polypeptides of protein, which confirmed some micro-environmental and conformational changes of HSA molecules. | 10-hydroxycamptothecin | albumin | Homo sapiens |
| 9 | The conformational investigation showed that the presence of HCPT decreased the alpha-helical content of HSA and induced the slight unfolding of the polypeptides of protein, which confirmed some micro-environmental and conformational changes of HSA molecules. | 10-hydroxycamptothecin | albumin | Homo sapiens |