Title : Reduction of the lipocalin type heme containing protein nitrophorin -- sensitivity of the fold-stabilizing cysteine disulfides toward routine heme-iron reduction.

Pub. Date : 2011 Nov

PMID : 21955842






2 Functional Relationships(s)
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1 In the present study, the effect of dithionite on the disulfides of NP4 and NP7 is addressed. Dithionite proteinase 3 Homo sapiens
2 With this background information, the effect of dithionite on the cystines of NP4 and NP7 was studied after trapping of the thiols with para-cloromercurybenzyl sulfonate (p-CMBS) and subsequent matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) where the heterolytic cleavage of the SS bond appears with only 2molar equivalents of the reductant. Dithionite proteinase 3 Homo sapiens