Title : Expression and characterization of UL16 gene from duck enteritis virus.

Pub. Date : 2011 Aug 24

PMID : 21861926






3 Functional Relationships(s)
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1 In this study, we analyzed the amino acid sequence of UL16 using bioinformatics tools and expressed in Escherichia coli Rosetta (DE3) induced by isopropy1-beta-D-thiogalactopyranoside (IPTG). Isopropyl Thiogalactoside UL16 Anatid alphaherpesvirus 1
2 The UL16 gene was cloned into a pET prokaryotic expression vector and transformed into Escherichia coli Rossetta (DE3) induced by IPTG. Isopropyl Thiogalactoside UL16 Anatid alphaherpesvirus 1
3 Anti-UL16 polyclonal sera was prepared by immunizing rabbits, and reacted with a band in the IPTG induced cell lysates with an apparent molecular mass of 60 kDa. Isopropyl Thiogalactoside UL16 Anatid alphaherpesvirus 1