Pub. Date : 2011 Jul 28
PMID : 21798060
5 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | RESULTS: The yield of isobutanol was improved from 0.16 to 0.97 mg per g glucose by simultaneous overexpression of biosynthetic genes ILV2, ILV3, and ILV5 in valine metabolism in anaerobic fermentation of glucose in mineral medium in S. cerevisiae. | isobutyl alcohol | ketol-acid reductoisomerase | Saccharomyces cerevisiae S288C |
| 2 | Overexpression of ILV6, encoding the regulatory subunit of Ilv2, in the ILV2 ILV3 ILV5 overexpression strain decreased isobutanol production yield by threefold. | isobutyl alcohol | ketol-acid reductoisomerase | Saccharomyces cerevisiae S288C |
| 3 | In aerobic cultivations in shake flasks in mineral medium, the isobutanol yield of the ILV2 ILV3 ILV5 overexpression strain and the reference strain were 3.86 and 0.28 mg per g glucose, respectively. | isobutyl alcohol | ketol-acid reductoisomerase | Saccharomyces cerevisiae S288C |
| 4 | CONCLUSIONS: Overexpression of genes ILV2, ILV3, ILV5, and BAT2 in valine metabolism led to an increase in isobutanol production in S. cerevisiae. | isobutyl alcohol | ketol-acid reductoisomerase | Saccharomyces cerevisiae S288C |
| 5 | This result implies that other constraints besides the enzyme activities for the supply of 2-ketoisovalerate may become bottlenecks for isobutanol production after ILV2, ILV3, and ILV5 have been overexpressed, which most probably includes the valine inhibition to Ilv2. | isobutyl alcohol | ketol-acid reductoisomerase | Saccharomyces cerevisiae S288C |