Title : Vasopressin-induced polyphosphoinositide and phosphatidylcholine degradation in fibroblasts. Temporal relationship for formation of phospholipase C and phospholipase D hydrolysis products.

Pub. Date : 1990 Oct 15

PMID : 2170380






4 Functional Relationships(s)
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1 Vasopressin-induced polyphosphoinositide and phosphatidylcholine degradation in fibroblasts. Phosphatidylcholines arginine vasopressin Rattus norvegicus
2 Here we demonstrate that VP-induced PIP2 hydrolysis is closely accompanied by phosphatidylcholine (PC) degradation by phospholipase D. Cells prelabeled with [3H]arachidonic acid showed rapid formation and diminution of [3H]diacylglycerol (DG) (5-15s) when treated with VP; this was accompanied by a reduction in polyphosphoinositide radioactivity. Phosphatidylcholines arginine vasopressin Rattus norvegicus
3 Here we demonstrate that VP-induced PIP2 hydrolysis is closely accompanied by phosphatidylcholine (PC) degradation by phospholipase D. Cells prelabeled with [3H]arachidonic acid showed rapid formation and diminution of [3H]diacylglycerol (DG) (5-15s) when treated with VP; this was accompanied by a reduction in polyphosphoinositide radioactivity. Phosphatidylcholines arginine vasopressin Rattus norvegicus
4 In cells prelabeled with [3H]myristic acid, which is predominantly incorporated into cellular PC, VP elicited the generation of [3H]myristoyl phosphatidate (PA) as early as 15 s, in the absence of an increase in labeled DG. Phosphatidylcholines arginine vasopressin Rattus norvegicus