Title : cAMP-dependent phosphorylation and inactivation of yeast transcription factor ADR1 does not affect DNA binding.

Pub. Date : 1990 Jun

PMID : 2161531






3 Functional Relationships(s)
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1 Transcription factor ADR1 increases the level of ADH2 gene expression 200-fold by binding to a palindromic upstream activation sequence (UAS1) in the glucose-repressible ADH2 promoter in Saccharomyces cerevisiae. Glucose DNA-binding transcription factor ADR1 Saccharomyces cerevisiae S288C
2 Intact ADR1 protein was detected at comparable levels in extracts made from repressed or derepressed yeast cells, indicating that glucose repression is not due to absence of ADR1. Glucose DNA-binding transcription factor ADR1 Saccharomyces cerevisiae S288C
3 ADR1 in extracts made from glucose-repressed and -derepressed cells bound UAS1 DNA with similar affinities despite having greatly different abilities to activate ADH2 gene expression in vivo. Glucose DNA-binding transcription factor ADR1 Saccharomyces cerevisiae S288C