Title : Xenopus transcription factor IIIA. Evidence for heterogeneity of Zn2+ binding affinities and specific labeling of cysteine 287.

Pub. Date : 1990 Aug 15

PMID : 2116409






4 Functional Relationships(s)
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1 The release of Zn2+ from transcription factor IIIA (TFIIIA) was examined with the metallochromic indicator 4-(2-pyridylazo)resorcinol (PAR) in the absence and presence of p-hydroxymercuriphenylsulfonate (PMPS). 4-(2-pyridylazo)resorcinol general transcription factor 3A L homeolog Xenopus laevis
2 The release of Zn2+ from transcription factor IIIA (TFIIIA) was examined with the metallochromic indicator 4-(2-pyridylazo)resorcinol (PAR) in the absence and presence of p-hydroxymercuriphenylsulfonate (PMPS). 4-(2-pyridylazo)resorcinol general transcription factor 3A L homeolog Xenopus laevis
3 With 0.5 mM PAR, approximately 5 eq of Zn2+ were released from TFIIIA, but no Zn2+ release was detected from the 7 S ribonucleoprotein. 4-(2-pyridylazo)resorcinol general transcription factor 3A L homeolog Xenopus laevis
4 The kinetic data of PAR and 5,5"-dithiobis-(2-nitrobenzoic acid) reactions with TFIIIA were similar, and the spectral changes were characterized by at least three exponential terms. 4-(2-pyridylazo)resorcinol general transcription factor 3A L homeolog Xenopus laevis