Title : Different kinetic properties of the two mutants, RAS2Ile152 and RAS2Val19, that suppress the CDC25 requirement in RAS/adenylate cyclase pathway in Saccharomyces cerevisiae.

Pub. Date : 1990 Jan 25

PMID : 2104846






4 Functional Relationships(s)
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1 By contrast, RAS2Ile152 shows a strong destabilization of the GDP complex (the dissociation rate constants of the RAS2Ile152.GDP complex is enhanced almost 50 times) and an increased GTPase activity. Guanosine Diphosphate Ras family GTPase RAS2 Saccharomyces cerevisiae S288C
2 By contrast, RAS2Ile152 shows a strong destabilization of the GDP complex (the dissociation rate constants of the RAS2Ile152.GDP complex is enhanced almost 50 times) and an increased GTPase activity. Guanosine Diphosphate Ras family GTPase RAS2 Saccharomyces cerevisiae S288C
3 Our kinetic results show that the functional modifications of RAS2 compensating for the CDC25 inactivation can not only be associated with the presence of a long-lived RAS2.GTP complex, but also with a rapid GDP to GTP exchange reaction. Guanosine Diphosphate Ras family GTPase RAS2 Saccharomyces cerevisiae S288C
4 Our kinetic results show that the functional modifications of RAS2 compensating for the CDC25 inactivation can not only be associated with the presence of a long-lived RAS2.GTP complex, but also with a rapid GDP to GTP exchange reaction. Guanosine Diphosphate Ras family GTPase RAS2 Saccharomyces cerevisiae S288C