Title : Evaluation of alternatives for human lysozyme purification from transgenic rice: impact of phytic acid and buffer.

Pub. Date : 2010 Sep-Oct

PMID : 20945487






5 Functional Relationships(s)
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1 In this study, we confirm that reducing phytic acid concentration improves lysozyme binding capacity and investigate alternative process conditions that reduce phytic acid interference. Phytic Acid lysozyme Homo sapiens
2 Compared with the previous best process, the adsorption capacity of human lysozyme was increased from 8.6 to 19.7 mg/mL when rice extract was treated with phytase to degrade phytic acid. Phytic Acid lysozyme Homo sapiens
3 Using tris buffer to adjust pH 4.5 extract to pH 6 before adsorption reduced phytic acid interference by minimizing phytic acid-lysozyme interactions, eliminated the need for phytase treatment, and increased the binding capacity to 25 mg/mL. Phytic Acid lysozyme Homo sapiens
4 Using tris buffer to adjust pH 4.5 extract to pH 6 before adsorption reduced phytic acid interference by minimizing phytic acid-lysozyme interactions, eliminated the need for phytase treatment, and increased the binding capacity to 25 mg/mL. Phytic Acid lysozyme Homo sapiens
5 Another method of reducing phytic acid concentration was to extract human lysozyme from rice flour at pH 10 with 50 mM NaCl in 50 mM sodium carbonate buffer. Phytic Acid lysozyme Homo sapiens