Title : Ex vivo generation of regulatory T cells: characterization and therapeutic evaluation in a model of chronic colitis.

Pub. Date : 2011

PMID : 20941602






1 Functional Relationships(s)
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1 We present a detailed protocol demonstrating that polyclonal activation of conventional CD4(+) T cells in the presence of IL-2, TGFbeta, and all trans retinoic acid induces >90% conversion of these T cells to Foxp3-expressing iTregs as well as promotes a three- to fourfold increase in proliferation following a 4-day incubation period in vitro. Tretinoin forkhead box P3 Mus musculus