Title : A prototypic microfluidic platform generating stepwise concentration gradients for real-time study of cell apoptosis.

Pub. Date : 2010 Apr 16

PMID : 20697588






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1 A sensitive apoptotic analysis method is integrated into this microfluidic system for studying apoptosis of HeLa cells under the influence of anticancer drug, etoposide, with various concentrations in parallel; it measures the yellow fluorescent proteincyan fluorescent protein fluorescence resonance energy transfer (FRET) signal that responds to the activation of caspase-3, an indicator of cell apoptosis. Etoposide caspase 3 Homo sapiens