Title : High-level expression and purification of Cys-loop ligand-gated ion channels in a tetracycline-inducible stable mammalian cell line: GABAA and serotonin receptors.

Pub. Date : 2010 Sep

PMID : 20662008






1 Functional Relationships(s)
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1 In a tetracycline-inducible stable human embryonic kidney cells (HEK293S) cell line, GABA(A) receptors containing alpha1 and beta3 subunits could be expressed with specific activities of 29-34 pmol/mg corresponding to 140-170 pmol/plate, the highest expression level reported so far. Tetracycline adrenoceptor alpha 1D Homo sapiens