Title : Enhancement of human prolactin synthesis by sodium butyrate addition to serum-free CHO cell culture.

Pub. Date : 2010

PMID : 20625486






4 Functional Relationships(s)
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1 Thus, the influence of NaBu on the production of recombinant human prolactin (hPRL) from CHO cells was investigated for the first time. sethoxydim prolactin Homo sapiens
2 Thus, the influence of NaBu on the production of recombinant human prolactin (hPRL) from CHO cells was investigated for the first time. sethoxydim prolactin Homo sapiens
3 Quantitative and qualitative analyses by reverse-phase high-performance liquid chromatography (RP-HPLC) and Western blot or SDS-PAGE, carried out directly on CHO-conditioned medium, showed that the highest hPRL expression was obtained with 1 mM NaBu. sethoxydim prolactin Homo sapiens
4 Our results show that NaBu increased the synthesis of recombinant hPRL in CHO cells, apparently without compromising either its structure or function. sethoxydim prolactin Homo sapiens