Title : Identification and characterization of the functional amino acids at the active center of pig liver thioltransferase by site-directed mutagenesis.

Pub. Date : 1991 Jul 5

PMID : 2061338






1 Functional Relationships(s)
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1 Seven oligonucleotides were designed, synthesized, and used to construct mutants, ETT-C22S, ETT-C25S, ETT-C25A, ETT-R26V, ETT-K27Q, ETT-R26V: K27Q, and ETT-C78S:C82S, by altering their codons in pig liver thioltransferase cDNA/M13mp18 clones. Oligonucleotides glutaredoxin-1 Sus scrofa