Title : Fast trypsin digestion of proteins on a cross-linked [Os(dmebpy)(2)Cl](+/2+)-derivatized copolymer of acrylamide and vinylimidazole column.

Pub. Date : 2010 Aug 15

PMID : 20607841






1 Functional Relationships(s)
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1 The digestion of reduced and alkylated bovine serum albumin and human transferrin in 2.5 mM ammonium bicarbonate and 0.2 M urea buffer was observed on the column, with more peptide coverage than conventional 4 h in-solution digestion at 37 degrees C. Control experiments without the Os-polymer in the column tip excluded the spontaneous in-solution digestion of proteins in the short time window of buffer delivery onto the column, indirectly confirming the contribution of Os-polymer on the fast trypsin digestion. Urea albumin Homo sapiens