Pub. Date : 2010 Jun
PMID : 20561442
4 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | [Effect of bortezomib on VEGF gene expression of endothelial cell line HMEC-1 and its possible mechanisms]. | Bortezomib | vascular endothelial growth factor A | Homo sapiens |
| 2 | This study was aimed to investigate the effects of bortezomib on VEGF gene expression of endothelial cell line HMEC-1, and to determine the changes of the transcriptional regulation activity of hypoxia-inducible factor 1 (HIF-1alpha) and expression intensity of Annexin A2, so as to analyze the possible mechanisms of the above expression of VEGF gene. | Bortezomib | vascular endothelial growth factor A | Homo sapiens |
| 3 | The results showed that after being treated by bortezomib with 2.5, 5.0, 10 nmol/L for 12 hours, the expression intensity of VEGF gene of endothelial cell line HMEC-1 was as follows: 0.730 +/- 0.106, 0.673 +/- 0.153, 0.767 +/- 0.090 (as 1.0 was made in 0 nmol/L) (p < 0.05); the proliferation activity of cells was not significantly suppressed by bortezomib in 2.5, 5.0 nmol/L (p > 0.05), while that was significantly suppressed by bortezomib of 10 nmol/L (p = 0.024), The results from RT-PCR showed that expression intensity of CA IX gene was conspicuously down-regulated by bortezomib in different concentrations, which suggested that the transcriptional regulation activity of HIF-1alpha was inhibited by bortezomib. | Bortezomib | vascular endothelial growth factor A | Homo sapiens |
| 4 | Bortezomib may down-regulate the expression of VEGF gene of endothelial cell through regulating the activity of HIF-1alpha and the expression of Annexin A2. | Bortezomib | vascular endothelial growth factor A | Homo sapiens |