Title : Epitope mapping in cell surface proteins by site-directed masking: defining the structural elements of NTPDase3 inhibition by a monoclonal antibody.

Pub. Date : 2010 Jul

PMID : 20511214






2 Functional Relationships(s)
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1 Using homology modeling, we built a 3D structure of NTPDase3 and designed 21 single cysteine mutations distributed over the surface of the enzyme. Cysteine ectonucleoside triphosphate diphosphohydrolase 3 Homo sapiens
2 Tethering NTPDase3 via cysteine residues located in a surface patch near the active site cleft masked the epitope and blocked antibody binding, as evaluated by enzyme inhibition assay and by ELISA. Cysteine ectonucleoside triphosphate diphosphohydrolase 3 Homo sapiens