Title : Dynamic quenching studies of fluorophore-labelled myosin subfragment 1 and its alkali light chain subunits.

Pub. Date : 1991 Apr 29

PMID : 2029538






1 Functional Relationships(s)
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1 The technique of fluorescence quenching by the non-ionic quenchers acrylamide and nicotinamide has been used to probe the accessibility of the environmentally sensitive N-(bromoacetyl)-N"-(1-sulpho-5-naphthyl) ethylenediamine (1,5-Br-AEDANS) fluorophore attached to either Cys-177 of the A1-light chain or the SH1 thiol (Cys-707) of the myosin subfragment (S1) heavy chain. Acrylamide myosin heavy chain 14 Homo sapiens