Title : A rapid purification method for neurogranin, a brain specific calmodulin-binding protein kinase C substrate.

Pub. Date : 1991 Apr 22

PMID : 2026258






1 Functional Relationships(s)
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1 This method takes advantage of the fact that the protein remains soluble in 2.5% perchloric acid (PCA) and that it binds to a calmodulin-Sepharose column in the absence of calcium: Other PKC substrate proteins that remain to be identified were also found to share these two properties, suggesting that a class of calmodulin-binding PKC substrates may exist in the brain. Sepharose calmodulin Bos taurus