Title : Directed evolution of recombinase specificity by split gene reassembly.

Pub. Date : 2010 Jul

PMID : 20194120






1 Functional Relationships(s)
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1 In this system, recombinase variants are selected for activity on new substrates based on enzyme-mediated reassembly of the gene encoding beta-lactamase that confers ampicillin resistance to Escherichia coli. Ampicillin beta-lactamase Escherichia coli