Title : Rapid release of N-linked glycans from glycoproteins by pressure-cycling technology.

Pub. Date : 2010 Mar 15

PMID : 20170179






1 Functional Relationships(s)
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1 With the application of pressure cycling from atmospheric to as high as 30 kpsi, >95% release of the asparagine-linked glycans from bovine ribonuclease B, human transferrin, and polyclonal human immunoglobulin was rapidly achieved in a few minutes using as low as 1:2500 enzyme:substrate molar ratio. asparagine-linked glycans transferrin Homo sapiens