Title : New function of the amino group of thiamine diphosphate in thiamine catalysis.

Pub. Date : 2009 Mar

PMID : 19364324






3 Functional Relationships(s)
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1 In this work, we investigated the rate of formation of the central intermediate of the transketolase reaction with thiamine diphosphate (ThDP) or 4"-methylamino-ThDP as cofactors and its stability using stopped-flow spectroscopy and circular dichroism (CD) spectroscopy. Thiamine Pyrophosphate transketolase Homo sapiens
2 In this work, we investigated the rate of formation of the central intermediate of the transketolase reaction with thiamine diphosphate (ThDP) or 4"-methylamino-ThDP as cofactors and its stability using stopped-flow spectroscopy and circular dichroism (CD) spectroscopy. Thiamine Pyrophosphate transketolase Homo sapiens
3 These data suggest that transketolase in the complex with the NH2-methylated ThDP exhibits dihydroxyethyl-4"-methylamino-ThDP-synthase activity. Thiamine Pyrophosphate transketolase Homo sapiens